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. 2021 Aug 17;9(8):1031. doi: 10.3390/biomedicines9081031

Figure 1.

Figure 1

The administration of AAV-mIL-30 reduced the number of CD25+CD4+ T cells and serum IFN-γ and IL-12 levels in ConA-induced hepatitis mice. Mice were injected with ConA for the induction of hepatitis. AAV-mock or AAV-mIL-30 were administered 5 days before ConA injection. Sera and liver tissues were collected at 4 h after ConA injection. (a) Serum levels of IL-30 were detected using ELISA. (b) Liver IL-30 mRNA expression was detected using RT-qPCR. Relative quantification was performed with the 2−ΔCT method. (c) The number of liver leukocytes was counted. (d) The level of CD25 expression on CD4+ T cells was analyzed using flow cytometry. (e) Serum levels of IFN-γ and IL-12 were detected. Each dot represents an individual mouse. n = 3–7 mice per group. All error bars denote ±SEM. *, p < 0.05; **, p < 0.01; ****, p < 0.0001.