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Ink4a and Arf expression marks Nepn-expressing cells. mESCs were induced to differentiate for 8 days as described, and cells expressing Nepn-expressing cells were isolated by FACS. Total RNA was extracted and analyzed by qPCR to check Ink4a, Arf, Nepn, and Oct4 gene expression. Data points represent the average of independent experiments performed in triplicate ± SD. *, p < 0.05.
