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. 2021 Aug 19;13(16):4165. doi: 10.3390/cancers13164165

Figure 4.

Figure 4

HDO and 2H-lactate production are directly related to [2H7]glucose consumption. Correlation plots of [2H7]glucose consumption vs. HDO production (top row) and 2H-lactate production (middle row) and correlation between 2H-lactate vs. HDO production (bottom row) are shown for the post-treatment period of A549 (Panels A,D and G), MiaPaCa2 (Panels B,E and H) and HCT-116 (Panels C,F and I). Correlations in all conditions were linear with R2 values > 0.96. Correlation plots were plotted from the calculated concentration of consumed [2H7]glucose and produced 2H-lactate and HDO by control and treated cells. The excellent linearity between 2H-lactate and HDO production and higher production of HDO demonstrates that the HDO could be a much more sensitive marker than lactate for monitoring NQO1 bioactivation.