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. 2021 Aug 8;13(16):3996. doi: 10.3390/cancers13163996

Figure 6.

Figure 6

The cisplatin conferring genes DUSP16 and SFN are significantly (>2 fold, p-value < 0.05) up-regulated in miR-200c-3p overexpressing cells. MMNK-1 cells were transfected with 10 nM mirVana miR-200c-3p mimic or 10 nM mirVana miRNA mimic Negative Control. After 48 h, total RNA was isolated from 70 to 90% confluent cells using QIAzol Lysis Reagent. Reverse Transcription Quantitative PCR (RT-qPCR) of cisplatin-resistance-conferring genes was performed, and gene expression was normalized to GAPDH and U6. Differences in gene expression were evaluated by the ΔΔCt method. Unpaired two-tailed t-test was used to evaluate statistical significance. * p < 0.05, ** p < 0.01, *** p < 0.001.