Figure 4.

Effects of XYP1 on the proliferation of T. gondii tachyzoites. HFFs were infected with tachyzoites and treated with two-fold serial dilutions of XYP1 and SFZ for 24 (A) or 48 h (C) to evaluate their effects on the tachyzoite proliferation. Infected cells were incubated with DMEM or XYP1 for 24 h (B) or 48 h (D), and then observed by the light microscope. The number of tachyzoites per PV was also calculated to evaluate their anti-tachyzoite effects (E,F). The means were determined by values obtained from three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 in comparison with negative control. Scale bars = 5 μm.