Potential therapeutic approaches based on TNTs. TNTs have been observed in several types of cancer, where they emerge to steer a more malignant phenotype thanks to the exchange of intracellular materials. (A) The design of molecules able to inhibit the formation of TNTs (i.e., inhibitors of actin polymerization, inhibitors of DNA synthesis, or inhibitors of mTOR pathway) may be a new therapeutic opportunity. (B) Since TNTs are long membrane nanotubes connecting distant cells, they can represent an exciting opportunity to deliver drugs or drug delivery systems in order to target inaccessible brain regions where GSCs are hidden and unreachable. Abbreviations: TNTs, tunneling nanotubes; GSCs, glioblastoma stem cells; GBM, glioblastoma.