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. 2021 Aug 9;10(8):2035. doi: 10.3390/cells10082035

Figure 1.

Figure 1

Hepatic insulin resistance expands to the cardiovascular system: representative gels (A,D) and quantification of in vivo insulin signaling studies (B,C,E,F) performed in control and iLIRKO mice at 6 and 12 months of age. After 10 min of human insulin injection at 1 U/kg body weight, aorta artery and heart were removed. Protein extracts from aorta artery and heart were analyzed by Western blot with anti-IRβ, anti-phospho-AKT (Ser473), anti-phospho-p42/44 MAPK (Thr202/Tyr204), anti-phospho-p70S6K (Thr389) and anti-β-actin antibodies. A representative experiment of three is shown. Results are expressed as mean ± SEM. n = 3–6 per group. The statistical significance was represented as ** (p < 0.01) and **** (p < 0.0001) vs. saline control mice; $ (p < 0.05), and $$$$ (p < 0.0001) vs. saline iLIRKO mice.