Table 3.
HPLC-DAD validation parameters for the analysis of anthocyanins.
| Precision a | Linearity | Sensitivity | Recovery # | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Intraday (CV%) | Interday (CV%) | Linear Range (μg/mL) | R2 | LOD | LOQ | % (mean ± SD) |
|||
| (μg/mL) | (μg/g) | (μg/mL) | (μg/g) | ||||||
| CY | 2.44 | 5.79 | 0.5–10 | 0.9987 | 0.04 | 0.86 | 0.15 | 3.00 | 92.2 ± 3.6 |
| PL | 2.06 | 4.77 | 0.5–10 | 0.9951 | 0.07 | 1.49 | 0.25 | 5.00 | 88.4 ± 7.0 |
| PE | 3.25 | 4.17 | 0.5–10 | 0.9972 | 0.14 | 2.79 | 0.50 | 10.00 | 96.3 ± 6.4 |
CY: cyanidin-3-O-glucoside, PL: pelargonidin-3-O-glucoside, PE: peonidin-3-O-glucoside. a n = 5; # n = 3.