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. 2021 Aug 3;11(8):1145. doi: 10.3390/biom11081145

Figure 9.

Figure 9

Downregulation of GhRDUF4D weakened upland cotton resistance to V. dahliae. (A) Photobleaching of the phenotype by inoculation TRV::CLA; (B) pathogenetic phenotypes of TRV::0 and TRV::GhRDUF4D cotton plants; (C) observation of the diagonal plane of stem vascular bundle; (D) the V. dahliae recovery assays of TRV::0 and TRV::GhRDUF4D stems; (E) relative expression level of GhRDUF4D in TRV::0 and TRV::GhRDUF4D cotton plants; (F) dynamic disease index of TRV::0 and TRV::GhRDUF4D cotton plants after Vd991 infection; (G) relative fungal biomass of TRV::0 and TRV::GhRDUF4D cotton plants; and (H,I) ROS and callose deposition observation in TRV::0 and TRV::GhRDUF4D cotton leaves, bar = 200μm. Mean ± SE, n = 3. “*” and “**” indicate that the data were significantly different at the p values of <0.05 and <0.01, respectively.