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The scatter plot (A) indicating that the non-detect rate increases with average ΔCt by location, tissue type and gene. The boxplots showing the comparison of −ΔCt residues between observed expressions and non-detects, (B) if conventional imputation of setting the Ct of each non-detect to 40 was conducted, and (C) if expectation-maximization (EM) algorithm was used to estimate the Ct of each non-detect.
