Figure 4.

Volcano plots of data from label-free quantification of differentially abundant proteins (DAPs) in hypoxic (1% of O₂) (A) Ri-1 cells, (B) U2904 cells, and (C) U2932 cells when compared to physioxia (5% of O₂). Red dots represent upregulated proteins with fold change ≥ 1.5, while green dots represent downregulated proteins with fold change ≤ −1.5, p-value cutoff < 0.05. Generated in Perseus software. The complete list of DAPs with all details is placed in Table S2. (D) Venn diagram showing number of DAPs overlapping among Ri-1, U2904, and U2932 cell lines in hypoxia when analyzed versus physioxia. The list of shared proteins is provided in Table S3. (E) The table showing the number of DAPs commonly identified in each cell line when (i) hypoxia compared to physioxia, and (ii) hypoxia compared to normoxia.