Table 3.

Some examples of chlorophyllides purification by solvents.

Purification by Solvents	Sources	Yield	References
Membrane suspension was added to a mixture containing acetone (or methanol) and 0.1 M ammonium hydroxide. After being mixed and centrifuged, these combined supernatants was added to petroleum ether or n-hexane.	Plasma membranes and thylakoid membranes of Anucystis nidulans	N/A	[133]
Extracted diethyl ether/ethanol (1:1) and repeatedly washed with 20% ethanol (10 mM Tricine–NaOH, pH 8).	R. capsulatus CB1200 cultured in Tween 80 supplemented growth medium	Chlorophyllide a: 7 mg/L	[134]
Barley leaves were extracted in 80% acetone (acetone/50 mM Tricine–NaOH, pH 8, 80:20, v/v), and the suspension was repeatedly extracted with n-hexane and diethyl ether/ethanol (1:1). The suspension was then repeatedly washed with 20% ethanol (10 mM Tricine–NaOH, pH 8).	Barley leaves (H. vulgare L.)	Chlorophyllide a: 17 μg/g fresh weightChlorophyllide b: 5 μg/g fresh weight

N/A: not available.