FIG. 8.

A double-point mutation reduces homodimeric DNA binding of mGCNF. (A) Schematic representation of mutations (M1 to M7) introduced into the H12 region of the mGCNF LBD. All mutants were in vitro translated in equal amounts in reticulocyte lysate in the presence of [³⁵S]methionine. (B) Binding of mGCNF, mGCNF-H11, and the mGCNF point mutants to the XRE1 in EMSA. Lane 1 contains unprimed reticulocyte lysate. The double-point mutant mGCNF-M6 shows significantly reduced homodimeric DNA binding (lane 8), whereas monomer binding is not affected.