Figure 1.
PDE3A and PDE5 inhibition dose-dependently decreases platelet integrin activation and granule secretion. Washed platelets were activated with collagen-related peptide (CRP, 0.3 µg/mL) and platelet integrin αIIbβ3 activation (PAC-1: 65.68 ± 17.42%) and platelet α-granule secretion (CD62P: 72.74 ± 8.17%), dense-granule secretion (CD63: 20.36 ± 13.97%) were measured by flow cytometry (A). Dose-dependent decrease by PDE3A (B) and PDE5 (C) inhibition. Data in duplicate in the percentage normalised against the activated platelets without inhibitor are shown. Corresponding vehicle controls were included for every condition. Histograms: black represents control, red represents CRP, pink represents cilostazol (5 µM), and blue represents tadalafil (10 nM). Mean + S.D., n = 5–7, * p < 0.05, *** p < 0.001, **** p < 0.0001. Statistics: ordinary one-way ANOVA followed by Holm-Sidak’s multiple comparisons test. CRP, collagen-related peptide.