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. 2021 Aug 16;10(8):2098. doi: 10.3390/cells10082098

Figure 4.

Figure 4

The mediating role of MGAT5 to HG and CML effects on CD147 glycosylation. Quantitative assessment of MGAT5 mRNA levels in cells treated with HG (25 mM) or CML (2 µg/mL) with and without NFκB inhibitory peptide (15 µg/mL), RAGE antibody (20 µg/mL) (A), or MGAT5-specific siRNAs (B), using real-time PCR. (C) Representative image and quantification of CD147 protein glycosylation in cells treated with HG (25 mM) or CML (2 µg/mL) with and without MGAT5-specific siRNAs using Pierce™ Glycoprotein Stain. The graphs represent the means ± SD for 3 independent experiments. (* p < 0.05) for comparison with the corresponding control.