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. 2021 Aug 16;12(8):1249. doi: 10.3390/genes12081249

Figure 2.

Figure 2

Direct proof in whole-blood DNA from AdEPO mice. (A) Strategy for designing specific primer-probe pairs for the viral genome. “Pr” indicates a primer-probe pair. (B) Detection of direct proof using the TaqMan qPCR assay performed with whole-blood DNA from the control (n = 12) and AdEPO mice (n = 15). (C–G) Sanger sequence analysis of the pooled amplicon on the TaqMan qPCR assay using five primer-probe pairs; R.S. indicates a reference sequence that completely matches the amplicon over 40 nucleotides on any primer-probe pair. N.D.: not detected. *** p < 0.001.