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. 2021 Aug 16;12(8):1249. doi: 10.3390/genes12081249

Figure 5.

Figure 5

Detection of direct and indirect proof for a long time period. To detect direct and indirect proof, small amounts of whole blood were harvested from the tail tips of mice (n = 12) until 30 days after the injection. From approximately 50 μL of whole blood, DNA/RNA was extracted, and the TaqMan or TB Green qPCR assays were performed to detect direct and indirect proof. (A) Overview of the experiments in this section, (B–F) continuous detection of direct proof for 30 days by five primer-probe pairs using the TaqMan qPCR assay; “Pr” refers to a primer-probe, (G–J): detection of changes in gene expression as indirect proof using the TB Green qPCR assay. N.D.: not detected. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. pre-value.