Table 1. Primer and probe sets of rRT-PCR assays for the detection of the 2019 novel coronavirus diseases (COVID-19).
| Target | Gene | Primer | Sequence (5’→3’) | Genome location* | Limit of detection (copies/mL) |
| *The location on the reference genome, accession ID: EPI_ISL_402119, EPI_ISL_402120. | |||||
| Forward | CCCTGTGGGTTTTACACTTAA | ||||
| Target 1 | ORF1ab | Reverse | ACGATTGTGCATCAGCTGA | 13342-13460 | 203 |
| Probe | FAM-CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ1 | ||||
| Forward | TTCTTGCTTTCGTGGTATTC | ||||
| Target 2 | E | Reverse | CACGTTAACAATATTGCAGC | 26303-26391 | 664 |
| Probe | FAM-GTTACACTAGCCATCCTTACTGCGCTTCGA-BHQ1 | ||||
| Forward | GGGGAACTTCTCCTGCTAGAAT | ||||
| Target 3 | N | Reverse | CAGACATTTTGCTCTCAAGCTG | 28881-28979 | 667 |
| Probe | FAM-TTGCTGCTGCTTGACAGATT-BHQ1 | ||||
| Forward | GGTCATGTGTGGCGGCTC | ||||
| Target 4 | RdRp | Reverse | GCTGTAACAGCTTGACAAATGAAAG | 15438-15545 | |
| Probe | FAM-CTATATGTTAAACCAGGTGGAAC-BHQ1 | ||||