Table 2. Real-time RT-PCR. The open reading frame 1ab (ORF1ab) and the nucleoprotein N gene region of the virus were recommended for primers and probes.
| Item | Sequence |
| Note: Results reading: negative (no Ct value or Ct=40); positive (Ct value <37, considered positive); and suspicious (if Ct value is between 37 and 40, repeat testing is recommended). If Ct value <40 in repeat testing and distinctive peaks are observed in the amplification curve, the sample is considered positive; otherwise, the sample is considered negative. | |
| Target 1 (ORF1ab) | |
| Forward primer (F) | CCCTGTGGGTTTTACACTTAA |
| Reverse primer (R) | ACGATTGTGCATCAGCTGA |
| Fluorescent probes (P) | 5’-FAM-CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ1-3’ |
| Target 2 (N) | |
| Forward primer (F) | GGGGAACTTCTCCTGCTAGAAT |
| Reverse primer (R) | CAGACATTTTGCTCTCAAGCTG |
| Fluorescent probes (P) | 5’-FAM-TTGCTGCTGCTTGACAGATT-TAMRA-3’ |