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Binding to l-dopa C. neoformans melanin, Sepia melanin, and synthetic melanin was tested by ELISA using φ4B4. Binding of φ33 to l-dopa was used as a control. The experiment was done twice, with similar results. Inset shows the configuration of the ELISA used to detect melanin binding. OD 405, optical density at 405 nm. Ab, antibody.
