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. 2021 Aug 9;10(8):2036. doi: 10.3390/cells10082036

Figure 2.

Figure 2

Generation of vascularized cerebral organoids. (a) Schematic diagram of the procedure used to generate vascularized cerebral organoids. (b) Blood vessel clumps (black arrowheads) fused to neural organoids generated on day 5 in the co-culture of developing cerebral organoids. (c) CD31+ endothelial cells were observed on day 13 of vhCO development. (d) CD31+ endothelial networks generated under different FBS conditions. (f) The percentage of organoids succeed to generate vessels after co-culture in different FBS concentrations. (e) Quantification of vessels performed using AngioTool. Data are plotted as means ± standard deviation. ** p < 0.01; one-way ANOVA. (g) Dextran was perfused on vhCO. (f) Ratios of successful vascularization under various FBS concentrations.