Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 13;7(8):1338–1346. doi: 10.1021/acscentsci.1c00064

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

ST-assisted in situ creation of Siglec-7 high-affinity ligands on live cells. (a) One-pot synthesis of CMP-^FTMCNeu5Ac (5). (b) ST6Gal1-assisted incorporation of ^FTMCNeu5Ac onto the peripheral NK cells was probed with the resultant fluorescein signals and Siglec-7-Fc binding. (c) ST6Gal1-assisted incorporation of ^FTMCNeu5Ac or natural Neu5Ac onto NK-92MI cells was probed by Siglec-7-Fc. (d, e) ST6Gal1-assisted incorporation of ^FTMCNeu5Ac onto the NK-92MI or NK-92MI-S7^high cells was probed with the resultant fluorescein signals (d) and Siglec-7-Fc binding (e). (b–e) Bars represent the standard error of three biological repeats of samples. The significance was analyzed with the two-sided Student’s t test. (ns) not significant; (*) p < 0.05; (**) p < 0.01.