Figure 5.

CTRP9 overexpression inhibits TLR4/MyD88/NF-κB signaling. (A) Protein expression levels were determined using western blotting. (B) Quantification of western blotting from (A). (C) mRNA expression levels were determined using reverse transcription-quantitative PCR. (D) NF-κB binding activity was determined using an electrophoretic mobility shift assay. The arrow indicates the direction of sample travel during electrophoresis. Data are presented as the mean ± SD (n=5). ^*P<0.05 vs. control. ^#P<0.05 vs. H/R or Ad-GFP. CTRP9, C1q/TNF-related protein 9; TLR4, toll-like receptor 4; MyD88, myeloid differentiation primary response; Ad-GFP, adenovirus encoding green fluorescent protein; NSB, non-specific binding; F, free probe.