Figure 1.
(a) Schematic representation of experimental paradigm. STZ was administered on the 0th day. The memory assessment was carried out on the 61st day, and the neurometabolic analysis on the 63rd day following infusion of [1,6-13C2]glucose/[2-13C]-acetate. Memory of the mice after (b) ICV-STZ (3 mg/kg), (c) ICV-STZ (5 mg/kg) treatment. The left and right panels depict the recognition and discrimination indices, respectively. Mice were first trained with two identical objects for 5 min. The working memory of mice was evaluated 1 h after the training by replacing the first object with a novel one. The recognition and discrimination indices were calculated using equations (1) and (2), respectively. The bar represent the mean±SD of the groups, while symbols denote individual values.