Representative 1H-[13C]-NMR spectra of cortical extracts from (a) Control, and (b) ICV-STZ (5 mg/kg) treated mice. Mice were infused with [1,6-13C2]glucose, and the brain metabolism was arrested using a focused beam microwave irradiation system immediately at 7 min of the infusion. Cortical metabolites were extracted using ethanol extraction protocol, and the NMR spectra were recorded using 600 MHz NMR spectrometer. Abbreviation used are: AlaC3, alanine-C3; AspC3, aspartate-C3; Cre, creatine; GABAC2, γ-aminobutyric acid-C2; GABAC3, γ-aminobutyric acid-C3; GABAC4, γ-aminobutyric acid-C4; GluC3, glutamate-C3; GluC4, glutamate-C4; GlnC4, glutamine-C4; LacC3, Lactate-C3; NAA, N-acetyl aspartate.