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(a) Number of differentially expressed transcripts of human endothelial cells co-cultured with brain pericytes under dynamic and static conditions. The total number of differentially expressed transcripts are shown on top of the bars. Testing for differential gene expression was performed using the DESeq2 R/Bioconductor package.19 (b) The volcano plot identifies the total changes in the data set. Black: no change, Blue: downregulated genes, Red: upregulated genes.
