Figure 5.

~100X magnification of TUNEL positive apoptotic cells and microglial phagoptosis of non-apoptotic cells. Q344X knock-in mouse retinal sections at 6 weeks (A–D), 15 weeks (E–H), and 24 weeks of age (I–L) were TUNEL-labeled (red) and immunolabeled for IBA1 (green) and rhodopsin (blue). Nuclei were labeled with DAPI (white). ROS/IS, rod outer segments/inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Arrowheads (>) indicate apoptosing cells. Arrows indicates phagoptosis (aberrant phagocytosis of living cells by microglia). Scale bars = 10 µm.