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. 2021 Aug 9;9(8):980. doi: 10.3390/biomedicines9080980

Figure 3.

Figure 3

Inflammatory genes–gene expression. RT-qPCR of IL6 (A), IL1B (B), TNF (C), BDKRB1 (B1R) (D), BDKRB2 (B2R) (E), LEP (F) and LEPR (G) in SGHPL-4 cells treated with continuous or intermittent exposure to cortisol (100, 250, 500, 1000 nM) and vehicle (0 nM) in the medium. Data were compared by two-way ANOVA with Tukey multiple comparisons test. Interaction between (continuous or intermittent) exposure to cortisol and cortisol dose was found for B1R (p < 0.0001), B2R (p = 0.0066), and LEP (p < 0.0001). Data are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; #, p = 0.9356; for continuous exposure: a, p < 0.0001 vs. control; for intermittent exposure: x, p < 0.0001 vs. control. IL6, Interleukin 6; IL1B, Interleukin 1β; TNF, Tumor necrosis factor α; B1R, Kinin B1 receptor; B2R, Kinin B2 receptor; LEP, Leptin; LEPR, Leptin receptor. Cell treatment was repeated at least three times.