Skip to main content
. 2021 Aug 9;9(8):980. doi: 10.3390/biomedicines9080980

Figure 4.

Figure 4

Angiogenic genes–gene expression. RT-qPCR of VEGFA (A), PlGF (B), KDR (VEGFR-2) (C), sFLT1 (soluble VEGFR-1) (D), MMP2 (E), and MMP9 (F) in SGHPL-4 cells treated with continuous or intermittent exposure to cortisol (100, 250, 500, 1000 nM) and vehicle (0 nM) in the medium. Data were compared by two-way ANOVA with Tukey multiple comparisons test. Interaction between (continuous or intermittent) exposure to cortisol and cortisol dose was found for VEGFA (p < 0.0001), and MMP2 (p < 0.0001). Data are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; #, p = 0.0674; &, p = 0.0549; for continuous exposure: b, p < 0.05 and a, p < 0.0001 vs. control; for intermittent exposure: y, p < 0.05 vs. control. VEGFA, Vascular endothelial growth factor A; PlGF, Placental growth factor; KDR (VEGFR-2), Vascular endothelial growth factor receptor 2; sFLT1 (soluble VEGFR-1), soluble Vascular endothelial growth factor receptor 1; MMP2, Matrix metalloproteinase-2; MMP9, Matrix metalloproteinase-9. Cell treatment was repeated at least three times.