Figure 8.

Tube-like structures formation. Representative pictures of tube-like structures (A) and the quantification of total branching points (B) total loops (C), and total tubes (D) after a 5-h treatment of SGHPL-4 cells with vehicle (0 nM), cortisol (500 nM), and/or DBK (1 µM) in the medium. The quantification of tubes, loops, and branching points was performed using the WimTube Software (Wimasis, Munich, Germany). Data were compared by one-way ANOVA with Tukey multiple comparisons test; * p < 0.05, ** p < 0.01, # p = 0.0521. Data are presented as mean ± SEM. DBK, Des-Arg9-Bradykinin. Cell treatment was repeated at least three times.