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. 2021 Aug 3;10(8):1971. doi: 10.3390/cells10081971

Figure 2.

Figure 2

ERF85OE lines showed enhanced ethylene-mediated stem phenotypes. (a) GUS staining in two transgenic lines that carry a pPtERF85::GUS reporter. Transgenic reporter lines were treated for 10 h with either water (mock) or 100 µM ACC. (b) Stem cross-sections from WT, transgenic ethylene-insensitive trees (pLMX5::etr1-1; [13]), and ERF85OE lines. Trees were treated with 10 µM ACC for 14 days. Cross-sections (50 µM-thick) were stained with safranine and toluidine blue and magnified using a 20-fold objective. Arrows highlight the onset of a gelatinous (G)-layer in xylem fibers [14]. (c) Plant height of ERF85OE lines in response to water (mock) or 100 µM ACC treatment for 14 days. Bars represent the average height (+/− standard error) obtained from five biological replicates.