Skip to main content
. 2021 Aug 5;9(8):961. doi: 10.3390/biomedicines9080961

Figure 2.

Figure 2

Detection of transgene expression in transduced cell lines. (A) Detection of secreted human GM-CSF and human IFN-α in cell supernatants of 293T/ Mock and 293T/ LV-G2α/gB-transduced cells. n.d. = not detected; (B) 293T cells transduced with LV-G2α/gB and analyzed by flow cytometry showed expression HCMV/gB on the cell surface. Mock (dashed black line) and LV-G2α/gB transduced (purple line). Representative results from triplicate experiments. The mean fluorescence intensity (MFI) is indicated below; (C) 3T3 cells transduced with LV-DR4/fLuc and analyzed by flow cytometry showing expression of the HLA-DR dimer on the cell surface. Mock (dashed black line) and LV-DR4/fLuc transduced (blue line). Representative results from triplicate experiments. The mean fluorescence intensity (MFI) is indicated below; (D) analyses of luminescent signals in cell lysates obtained from 3T3 mock cells (black line) and 3T3 cells transduced with LV-DR4/fLuc (blue line).