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. 2021 Aug 26;9:144. doi: 10.1186/s40478-021-01244-0

Fig. 4.

Fig. 4

Gray matter hemoglobin leakage along the spinal cord axis. Immunohistochemical labelling (a) and automated analysis outputs (b) of extravascular hemoglobin in control and ALS spinal cord gray matter. Scale bars = 1 mm. Quantification of extravascular hemoglobin in gray matter at individual segmental levels C8, T7–T9 and L4/L5 in control (c) and ALS (d) cases. Data shown as mean ± SD (control n = 4, ALS n = 13), with statistical significance determined using two-way ANOVA with Tukey’s post-test. ns, not significant