Skip to main content
. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: FASEB J. 2021 Jun;35(6):e21653. doi: 10.1096/fj.202001921RR

FIGURE 3.

FIGURE 3

Expression of osteoclast-related proteins in wild type and Orai1fl/fl-LysMcre bone marrow and in macrophages with and without RANKL differentiation. A, In RNA from unstimulated whole marrow wild type (WT) and Orai1fl/fl-LysMcre (Orai1−/−) cells, expression of mRNA for a bone protein, osteprotegerin (OPG), a bone produced osteoclast differentiation protein (RANKL) and bone resorption-related proteins of osteoclasts, cathepsin K, TRAP, and ATPa3, relative to GAPDH. In whole marrow, differences were statistically significant for TRAP and RANKL at P < .02. N = 4, mean ± SD. B, Expression of TRAP relative to GAPDH determined by qPCR analysis of mRNA from control (WT) and Orai1fl/fl-LysMcre (Orai1−/−) bone marrow cells, depleted of stromal cells, and cultured with mCSF without (left) or with RANKL (right) for 7 days (N = 3, mean ± SD). Because data showed a non-normal distribution, results were analyzed by non-parametric ANOVA (Kruskal-Wallis test) with Dunn's test for multiple comparisons; TRAP expression was significantly greater (P < .01, N = 3) in the RANKL-treated cells from Orai1fl/fl-LysMcre animals compared to cells from controls with and without RANKL. C, Expression of osteoclast markers in RANKL-treated cultured marrow cells from Orai1fl/fl-LysMcre (Orai1−/−) animals determined by qPCR; results expressed as fold change compared to controls (WT); mean ± SD, N = 3. DC-STAMP is significantly reduced in the absence of Orai1 (P < .001), while ATP6v0d2 shows a significant increase (P < .01)