Figure 5.

Immunohistochemical analysis for Iba1. The upper panel shows an overview slice with Iba1 and DAPI expression. The tumor mass (T) is delineated by a yellow line. Around the tumor mass, a peritumoral area (PT) was delineated by a yellow dashed line. The lower panel shows ROIs in the different zones. T: ROI in the tumor mass with Iba1 staining; infiltration of granular Iba1-positive cells can be observed. PT: ROI in the peritumoral region with Iba1 staining; numerous Iba1-positive cells with more processes were found around the tumor mass. C: ROI in the contralateral healthy brain tissue with Iba1 staining; Iba1-positive cells are identified, but less present compared to the tumor mass and especially the peritumoral region. T—tumor mass; PT—peritumoral region; C—contralateral healthy brain tissue.