Table 4.
Translational optimal codons of G. duodenalis.
| AA | Codon | High RSCU (N) | Low RSCU (N) | AA | Codon | High RSCU (N) | Low RSCU (N) |
|---|---|---|---|---|---|---|---|
| Phe | UUU | 0.36 (632) | 1.24 (1846) | Ser | UCU | 0.88 (972) | 1.58 (3140) |
| UUC * | 1.64 (2845) | 0.76 (1132) | UCC * | 1.66 (1833) | 0.75 (1484) | ||
| Leu | UUA | 0.07 (94) | 0.99 (1832) | UCA | 0.27 (301) | 1.20 (2392) | |
| UUG | 0.27 (377) | 0.96 (1777) | UCG * | 1.13 (1249) | 0.53 (1044) | ||
| CUU | 0.96 (1350) | 1.31 (2421) | AGU | 0.32 (353) | 0.98 (1943) | ||
| CUC * | 2.96 (4153) | 0.76 (1399) | AGC * | 1.74 (1929) | 0.96 (1915) | ||
| CUA | 0.14 (190) | 1.06 (1962) | Pro | CCU | 0.64 (807) | 1.21 (1684) | |
| CUG * | 1.61 (2262) | 0.92 (1690) | CCC * | 1.57 (1974) | 0.76 (1063) | ||
| Ile | AUU | 0.51 (801) | 1.16 (2187) | CCA | 0.38 (480) | 1.43 (1992) | |
| AUC * | 2.16 (3418) | 0.73 (1369) | CCG * | 1.41 (1778) | 0.59 (824) | ||
| AUA | 0.33 (529) | 1.11 (2088) | Thr | ACU | 0.50 (729) | 1.21 (2058) | |
| Met | AUG | 1.00 (2296) | 1.00 (2331) | ACC * | 1.30 (1914) | 0.77 (1306) | |
| Val | GUU | 0.60 (1048) | 1.13 (1611) | ACA | 0.62 (917) | 1.42 (2413) | |
| GUC * | 2.27 (3977) | 0.74 (1066) | ACG * | 1.58 (2319) | 0.60 (1013) | ||
| GUA | 0.17 (297) | 1.11 (1590) | Ala | GCU | 0.52 (1271) | 1.22 (2344) | |
| GUG | 0.96 (1687) | 1.02 (1461) | GCC * | 1.70 (4180) | 0.77 (1487) | ||
| Tyr | UAU | 0.40 (638) | 1.21 (1682) | GCA | 0.63 (1551) | 1.47 (2826) | |
| UAC * | 1.60 (2521) | 0.79 (1095) | GCG * | 1.15 (2823) | 0.54 (1042) | ||
| His | CAU | 0.32 (338) | 1.13 (1582) | Cys | UGU | 0.32 (702) | 1.05 (1125) |
| CAC * | 1.68 (1779) | 0.87 (1221) | UGC * | 1.68 (3650) | 0.95 (1015) | ||
| Gln | CAA | 0.21 (335) | 1.07 (2489) | Trp | UGG | 1.00 (823) | 1.00 (749) |
| CAG * | 1.79 (2878) | 0.93 (2171) | Arg | CGU | 0.57 (547) | 0.87 (1036) | |
| Asn | AAU | 0.39 (763) | 1.15 (2189) | CGC * | 2.58 (2471) | 0.75 (890) | |
| AAC * | 1.61 (3105) | 0.85 (1618) | CGA | 0.25 (242) | 0.93 (1105) | ||
| Lys | AAA | 0.18 (548) | 0.92 (2284) | CGG | 0.91 (868) | 0.89 (1056) | |
| AAG * | 1.82 (5508) | 1.08 (2680) | AGA | 0.37 (355) | 1.57 (1859) | ||
| Asp | GAU | 0.45 (1310) | 1.23 (3040) | AGG * | 1.32 (1264) | 0.99 (1168) | |
| GAC * | 1.55 (4509) | 0.77 (1913) | Gly | GGU | 0.41 (833) | 0.96 (1045) | |
| Glu | GAA | 0.23 (721) | 1.00 (2804) | GGC * | 1.87 (3831) | 0.91 (987) | |
| GAG * | 1.77 (5474) | 1.00 (2795) | GGA | 0.54 (1106) | 1.30 (1407) | ||
| GGG * | 1.18 (2417) | 0.83 (898) |
Comparison of codon usage frequency between high and low expression genes of G. duodenalis. The optimal codons were determined by a Chi-square contingency test. * indicates that the frequency of the codons is much higher (p < 0.01). AA, amino acid; N, number of codons.