FIG. 8.

Stability of the IRE–IRP-1 complexes. ³²P-labelled IRE.34, IRE.100, or IRE.ORF probes (2 ng) were incubated with 100 ng of recombinant IRP-1 in 40% RRL for 10 min at 30°C, to allow formation of IRE-IRP complexes, before addition of IRE competitor (1 μg) (lanes 1 to 4) or 1 μg of rRNA (lanes 5 to 8). Aliquots were taken after 0, 10, 20, and 60 min and analyzed on a running nondenaturing gel. In control reactions, the IRE competitor RNA was added simultaneously with the probe (lanes 9 to 12).