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. 2021 Aug 13;22(4):1175. doi: 10.3892/etm.2021.10607

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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AMPK regulates the expression of Hpa-1 and TJ proteins. (A) Western blot analysis of Hpa-1, Sdc-1, occludin-1 and ZO-1 expression. Densitometric analysis of (B) Hpa-1 and (C) Sdc-1 expression. (D) Expression levels of Sdc-1 in the cell culture supernatant. (E) Densitometric analysis of occludin-1 and (F) ZO-1 expression. Data are presented as the mean ± standard deviation of three repeats. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. the control group; ^#P<0.05, ^##P<0.01 vs. HG. AMPK, adenosine monophosphate-activated protein kinase; Hpa-1, heparanase-1; TJ, tight junction; Sdc-1, syndecan-1; ZO-1, Zonula occludens-1; HG, high glucose.