Table 3.

The gradient elution conditions of the mobile phase

Time (min)	Phase A (%)	Phase B (%)	Phase C (%)
0–15	5–17	0–83	95–0
15–24	17–20	83–80	0
24–48	20–24	80–76	0
48–52	24–28	76–72	0
52–60	28–31	72–69	0
60–65	31–35	69–65	0
65–80	35–70	65–30	0
80–81	70–5	30–0	0–95
81–90	5	0	95