Figure 1.

Effects of insulin and GA on the expression of Mg²⁺ transporters. The cDNA was prepared from total RNA of NRK-52E cells treated with vehicle (CNT), 10 nM insulin, or 500 μg/mL GA for 6 h. Real time PCR was carried out by primers for TRPM6, TRPM7, CNNM2, and β-actin. The expression levels of TRPM6, TRPM7, and CNNM2 were normalized by β-actin, and were represented as percentage of CNT. n = 3–4. ** p < 0.01 and ^NS p > 0.05 compared with CNT.