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. 2021 Aug 11;22(4):1160. doi: 10.3892/etm.2021.10594

Figure 3.

Figure 3

Overexpression of miR-30a-5p reduces the concentration of the inflammatory factors in LPS-induced MLE-12 cells. (A) The mRNA expression level of miR-30a-5p in the MLE-12 cells was detected using RT-qPCR. **P<0.01 vs. control. (B) The mRNA expression level of miR-30a-5p following transfection with miR-30a-5p mimics/inhibitor/NC into the MLE-12 cells was detected using RT-qPCR. (C) The viability of the MLE-12 cells was measured using a MTT assay. The concentration of (D) IL-6, (E) IL-1β and (F) TNF-α following transfection with miR-30a-5p mimics/NC into the MLE-12 cells was measured using ELISA. **P<0.01 vs. miR-NC. ##P<0.01 vs. inhibitor NC. NC, negative control; miR, microRNA; LPS, lipopolysaccharide; TNF, tumor necrosis factor; RT-qPCR, reverse transcription-quantitative PCR.