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. 2021 Aug 11;22(4):1160. doi: 10.3892/etm.2021.10594

Figure 5.

Figure 5

lncRNA HOTAIR knockdown decreases the concentration of the inflammatory factors in LPS-induced MLE-12 cells by regulating the miR-30a-5p/PDE7A axis. (A) The protein expression level of PDE7A in the MLE-12 cells was determined using a western blot assay. **P<0.01 vs. control. (B) The protein expression level of PDE7A following transfection with pcDNA-PDE7A/NC into the MLE-12 cells was determined using western blot analysis. **P<0.01 vs. pcDNA-NC. (C) The protein expression level of PDE7A following transfection with sh-HOTAIR + miR-30a-5p inhibitor/sh-HOTAIR/NC into the MLE-12 cells was determined using western blot analysis. **P<0.01 vs. sh-NC. ##P<0.01 vs. sh-HOTAIR. (D) The viability of the MLE-12 cells was measured using a MTT assay. The level of (E) IL-6, (F) IL-1β and (G) TNF-α following transfection with sh-HOTAIR + miR-30a-5p inhibitor/sh-HOTAIR + pcDNA-PDE7A/sh-HOTAIR/NC into the MLE-12 cells was measured using ELISA. **P<0.01 vs. sh-NC. ##P<0.01 vs. sh-HOTAIR. NC, negative control; miR, microRNA; LPS, lipopolysaccharide; TNF, tumor necrosis factor; sh, short hairpin.