Figure 6.

lncRNA HOTAIR knockdown attenuates LPS-induced ARDS and ARDS-related inflammatory response in vivo. The mRNA expression level of (A) HOTAIR, (B) miR-30a-5p and (C) PDE7A in the lung tissues of mice was detected using reverse transcription-quantitative PCR. (D) Histopathological change and injury score were detected using H&E staining. Magnification, x200. (E) Lung wet/dry weight ratio, (F) PaO₂ and (G) PaCO₂ was measured using an automatic blood gas analyzer. The concentration of (H) IL-6, (I) IL-1β and (J) TNF-α in BALF of mice was measured using ELISA. ^**P<0.01 vs. sham. ^##P<0.01 vs. ARDS. Pa, partial pressure; TNF, tumor necrosis factor; miR, microRNA; NC, negative control; sh, short hairpin; ARDS, acute respiratory distress syndrome.