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. 2021 Aug 13;10(8):2087. doi: 10.3390/cells10082087

Figure 6.

Figure 6

PODXL overexpression accelerated EMT progression and metastatic ability in CRC cells. (a) SW480 cells were transfected with control vector (pCMV6-XL3) or PODXL and then exposed to IR (5 Gy). Twenty-four hours after transfection, cell migration and invasiveness were determined using a transwell assay. The graph indicates relative cell numbers from three independent experiments. Scale bar, 100 μm. and *** p < 0.005. (b) pCMV6-XL3- or PODXL-expressing SW480 cells were assessed by Western blot for PODXL, ZO1, and p-FAK expression. The β-actin signal served as loading control. *** p < 0.005. (c) IF staining of PODXL (red) and ZO1 (green) in pCMV6-XL3- or PODXL-expressing SW480 cells. Nuclei were counterstained with DAPI. Scale bar, 100 μm. The graph shows fluorescence intensity ± SD. *** p < 0.005.