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Thiorphan inhibition assay. SH-SY5Y cells were incubated for 12 h in the presence or not of compound 4 or 19 (5 μM), followed by thiorphan (50 μM) for 30 min before Aβ-degrading activity was measured using the appropriate substrate. The data are the mean ± SD for three independent samples. * p < 0.05; *** p < 0.001; ****, p < 0.0001 compared to the control by one-way ANOVA.
