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. 2021 Aug 27;13:134. doi: 10.1186/s13073-021-00950-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Effect of sequencing depth on de novo genome assembly. a Sequencing depth of samples from Korea, Japan, and India. Red data points, nine samples used for the generation of simulated samples for different sequencing depths. b Total read length of samples from Korea, Japan, and India. c The average number of genomes (left axis) and the proportion of (HQ + NC) genomes (right axis) from nine samples. d, e Completeness (d) and N50 (e) of assembled genomes from lower sequencing depth (left box of each column) and greater sequencing depth (right box of each column). f The number of the assembled genomes from Korea, Japan, and India. g Total number of the assembled genomes from Korea, Japan, and India, and genome assembly yields. h The relative abundance of 224 Korea-specific, 338 Japan-specific, and 18 India-specific assembled genomes in independent fecal samples from the USA (n = 926). P values were calculated by two-sided Mann–Whitney U test (**: P < 0.01; ***: P < 0.001).