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. 2021 Aug 19;10(8):2137. doi: 10.3390/cells10082137
LF and RF left and right flanking sequences homologous to the intergenic region upstream the locus of HTR5 gene;
P1 P-NOS promoter of A. tumefaciens nopaline synthase gene;
P2 CaMV35S promoter of cauliflower mosaic virus;
nptII neomycin phosphotransferase II gene, which provides plant cell resistance to kanamycin;
S DNA sequence encoding the leader signal of the carrot extensin gene, which ensures the transport of deltaferon into the apoplast;
dIFN DNA sequence encoding the target dIFN protein;
GST DNA sequence encoding a tag for affinity protein purification;
sgRNA Cas9 endonuclease recognition sites homologous to the site in the intergenic region upstream the A. thaliana HTR5 gene for excision of the construct from the plasmid in the cell.