FIG. 2.

Analysis of transcription from the naked DNA template and from the nucleosome-reconstituted array template with or without GAL4 derivatives. (A) Transcripts detected by primer extension from the E4 promoter (E4) of naked template DNA (pIC2085S/G5E4R) and that from the HIV promoter (HIV) of control plasmid pHIV(D,N) are indicated on the left. A 12.5 nM concentration of DNA-binding activity of each GAL4 derivative was included where indicated. Lane 1 is a control reaction to which no GAL4 derivatives were added. Lanes 6 to 13 show transcripts in the presence of HATs (SAGA and NuA4) and acetyl-CoA. (B) Transcripts from the G5E4-5S nucleosome array template in the presence or the absence of acetyl-CoA without or with the same amounts of various GAL4-derivative proteins as used for panel A.