Figure 2.

The effects of rMSA on the function of skeletal muscles in mice (a,b) The grip strength of female (a) and male (b) mice. (c–e) The muscle weight of female + male (c), female (d), and male (e) mice. (f) The Toluidine Blue O staining of gastrocnemius muscle. Scale bar, 50 μm. (g,h) The cross-sectional area of myofibers in female (g) and male (h) mice. (i) The immunofluorescence staining for MYH7 (green) and DAPI (blue) in mice. Scale bar, 50 μm. (j,k) The relative level of MYH7 in female (j) and male (k) mice. Mice were treated with rMSA 1.5 mg per gram of body weight or isometric saline every 3 weeks for 8 months. All graphs represent mean with SEM, with p values calculated by the two-tail t test. n, number of mice used for each analysis.