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. 2021 Aug 6;10(8):2010. doi: 10.3390/cells10082010

Figure 3.

Figure 3

Deletion of HMGB1 shifts the balance away from autophagy activation and toward a pro-inflammation state. (a) Immunoblot analysis of p-ULK1 at Serine 757 in B6 mice to evaluate its expression level changes over a time course of 2 h, 4 h, 1 d, 3 d, and 7 d post RD. (b) Graph shows the quantification of protein levels based on densitometry of the Western blots in (a). (c) Immunoblot analysis of p-ULK1 at Serine 757 and 555, and of p-ATG14 at Serine 29 in HMGB1Δrod and littermate control mice at 4 h and 3 d post RD. (d–f) Graphs show the quantification of protein levels based on densitometry of the Western blots in (c). Naïve retinas without manipulation of either eye were used as attached controls. GAPDH serves as a loading control. CTL: control, Δrod: HMGB1Δrod. Data are from three independent experiments and plotted with mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001 (One-way ANOVA). Stars on top of columns represent statistical results compared to the control naïve group.